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By Rodolfo Paoletti, Dr. David Kritchevsky

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2137 gm/ml). Again, pipetting errors as well as differences in protein and lipoprotein content of the serum will lead to small variations of this background salt solution. 3 rotor at 40,000 rpm for 24 to 26 hours, with rotor temperature maintained at 16° to 18°C. This time of 30 E W I N G E T AL. fractionation is a minimum time in which essentially quantitative re­ covery of the high-density lipoprotein spectra may be accomplished. 15 gm/ml. 5-ml fraction is pipetted as a background salt reference.

The results of lipid analyses of the separated lipoprotein fractions are, in general, consistent with earlier composition data of Hillyard et al. (27), Lindgren et al. (28), and Bragdon et al. (25), except that a down­ ward revision is indicated for the triglyceride content of both Sf 0-20 and HDL fractions. In Sf 0-20 the compensating percentage increase is distributed between cholesteryl esters and phospholipids, whereas in HDL there is a substantially larger value for cholesteryl esters only.

At frame boundaries, 1 is approximated by the X in the adjacent interval. Then: Ci = Ci i = 0,2,4,.... and C« = Ci_i + dH i = 1, 3, 5, The expanded integral curve consists of these d paired with F* and X*. Using the tables of C and X values, m% can be calculated for each standard interval by linear interpolation: the desired value will corre­ spond to the concentration (Ci + Ci_i)/2. This permits the standard cor­ rection to base of cell for each component (15) according to: dBC = d (mi/xo)2 Before output, the CiBG are divided by an appropriate factor that relates them to the original lipoprotein sample.

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