By M. Ahearne, Alicia J. El Haj, S. Rauz (auth.), Alicia El Haj, Dan Bader (eds.)
This quantity offers chosen peer-reviewed papers of the eighth overseas convention on mobile & Stem phone Engineering (ICCM) 2010 in Dublin. The contributions are written by way of prime scientists in mobile and Stem telephone Engineering and the subjects of the papers comprise:
Computational cellphone Mechanics
Experimental strategies in cellphone Mechanics
Molecular and phone Imaging
Cell Matrix Interactions
Mechanotransduction and mobile mechanics
Stem mobilephone area of interest
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Extra resources for 8th International Conference on Cell & Stem Cell Engineering (ICCE)
In addition they have been shown to provide a well characterized mechanical environment (3), suitable for investigating cellular responses to biophysical stimuli. The biosynthetic activity of chondrocytes during in vitro cultivation is known to depend on both the biochemical and biophysical stimuli experienced by the cells. Previous studies have shown that the application of dynamic compressive loading to chondrocyte-seeded agarose hydrogels enhances cartilage specific matrix synthesis (4-6). However a recurring problem with these agarose constructs is the heterogeneous deposition of ECM within them, with typically greater matrix accumulation in the peripheral regions of the construct (7).
Different architecture with same culturing conditions in same region IV. DISCUSSION The purpose of this study was to investigate the influence of dynamic compressive loading and modified construct architecture on the in vitro development of engineered cartilage tissue. It has been previously shown that the biosynthetic activity of chondrocytes depends on the biophysical stimuli experienced by the cells (5). We also observed greater sGAG accumulation in loaded solid constructs compared to all other groups, but we did not find that loading enhanced matrix accumulation in DCM groups.
Fig. 2 Analysis of hESCs after 13 passages. A: hESCs cultured on Matrix showed increased population doublings compared to hESCs cultured on Matrigel. B: hESCs cultured on either Matrigel or Matrix showed defined colony edges and high cytoplasm to nucleus ratio. The latter hESCs grew in smaller colonies than the former. Arrows indicate examples of spontaneous differentiation. Bar 500um. C: hESCs cultured on either Matrigel or Matrix were stained for various pluripotency markers and the positive percentage of population were plotted, showing no obvious difference in pluripotency levels.